Acute Lymphoblastic Leukaemia (ALL)

Acute lymphoblastic leukemia (ALL) is an acute form of blood cancer characterized by overproduction of immature lymphoid cells which proliferate and accumulate in the bone marrow, preventing it from making normal blood cells. ALL is the most common type of cancer and leukemia in children. ALL progresses quickly and would probably be fatal within a few months without treatment[1-2]. The type of treatment depends on a number of factors including the subtype of ALL and the genetic make-up of the leukaemic cells.

BCR-ABL1 fusion (also known as Philadelphia chromosome/ translocation) which resulted from a fusion/ rearrangement between BCR gene on chromosome 22 and ABL1 on chromosome 9 is found in about 25% of ALL cases. Another 25% of ALL, known as Philadelphia chromosome-like ALL (Ph-like ALL), have an RNA expression pattern similar to that of BCR-ABL1 positive but lack the BCR-ABL1 fusion, other fusion genes are present instead. Identification of BCR-ABL1 positive ALL and Ph-like BCR-ABL1 negative ALL is critical as both respond to TKIs.

Oncode ALL panel offers PCR and NGS assays to detect BCR-ABL1 fusion and other fusions implicated in ALL.

ALL Fusion (RNA Assays)

ONCODEduce ALL BCR/ABL1 Major & Minor PCR Panel
ONCODEduce ALL BCR/ABL1 Major & Minor PCR Panel is a qualitative RT-PCR assay to detect the Major (e13a2/b2a2 & e14a2/b3a2) & Minor (e1a2) subtypes. Other transcript subtypes will not be detected. Amplicon identification is by fragment analysis.

Specimen Requirements:
Blood: 3ml in EDTA OR Marrow Aspirate: 1-2.5ml in EDTA

Turn Around Time:
• 10 working days

Shipment Requirement:
• Keep specimen at room temperature. DO NOT expose to direct sunlight.
ONCODEduce ALL Fusion Comprehensive NGS Panel
ONCODEduce ALL Fusion Comprehensive NGS Panel is designed to detect almost all fusions implicated in ALL by targeting 87 genes including BCR/ABL1 and those to identify Ph-like BCR/ABL1 negative B-ALL.

Specimen Requirements:
Blood: 3ml in EDTA OR Marrow Aspirate: 1-2.5ml in EDTA OR Aspirate: 2 smears with adequate cellularity

Turn Around Time:
• 15 working days

Shipment Requirement:
• Keep specimen at room temperature. DO NOT expose to direct sunlight.
References:
1. American Cancer Society (2017). About Acute Lymphocytic Leukemia. READ MORE
2. Brown, V., Borinstein, S. & Friedman, D. (2016). Molecular Profiling of Acute Lymphoblastic Leukemia. My Cancer Genome. READ MORE