PCR and Fragment Analysis

Polymerase Chain Reaction (PCR) is a technique used to amplify a specific piece of DNA or RNA from a sample. PCR can be used to detect certain chances in gene or chromosome. Fragment analysis is a genetic marker analysis which rely on the detection of the changes in the length of a specific DNA sequence to indicate the presence of absence of a genetic marker[4].

How does Fragment Analysis Works? [4]

1. Lovly, C., Berger, M. & Vnencak-Jones, C. (2016). Circulating tumor DNA. My Cancer Genome. Available HERE
2. National Cancer Institute (2017). Liquid biopsy: Using DNA in blood to detect, track, and treat cancer. National Cancer Institute. Available HERE
3. Behjati, S., & Tarpey, P. S. (2013). What is next generation sequencing? Archives of Disease in Childhood. Education and Practice Edition, 98(6), 236–238. LINK
4. The Tayside Centre for Genomic Analysis (2014). Fragment analysis. Available HERE
5. MRC-Holland-MLPA- an introduction. Available HERE