ONCODEcipher BRAF PCR Panel
PCR method to detect BRAF mutation at V600E hotspot in all solid tumours especially in melanoma to provide treatment options.
Gene Covered -- BRAF V600E
Polymerase Chain Reaction (PCR)
DNA extracted from FFPE tissue is subjected to PCR with primers targeting the hotspot region & amplicons are separated and identified by agarose gel electrophoresis.
FFPE tissue (FFPE block or 5 unstained slides [10µm thickness] with minimal surface area of 5mm2 and at least 30% tumour cellularity) & 1 H&E stained slide
Turn Around Time:
10 working days
Keep specimen at room temperature. DO NOT ship on ice or dry ice or expose to direct sunlight.