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ONCODEcipher Colon Essential67 + MSI Panel

Product Description:

FFPE DNA panel targeting 67 relevant genetic mutations in colon cancer AND microsatellite instability (MSI) status to provide treatment options & prognostic indications.

Highlighted Genes -- Mutations of RAS/ RAF/ MEK/ MAP kinase signalling pathway


Next Generation Sequencing (NGS) AND
Polymerase Chain Reaction (PCR) & Fragment Analysis

Test Methodology:

Essential 67 NGS Panel
DNA extracted from FFPE tissue is subjected to amplification-based library preparation and massively parallel sequencing. Sequencing analysis is performed using commercial clinical bioinformatics pipelines and annotation is done using proprietary and public databases.

MSI PCR & Fragment Analysis Panel
DNA extracted from FFPE tissue (tumour) & blood (non-tumour) is subjected to PCR & amplicons are separated and identified by fragment analysis. The pattern of amplicons for the 5 mononucleotide markers is compared between tumour and non-tumour.

Specimen Requirements:

FFPE tissue (FFPE block or 10-15 unstained slides [10µm thickness] with minimal surface area of 5mm2 and at least 30% tumour cellularity) & 1 H&E stained slide
AND 3ml Blood in EDTA tube(s)


Turn Around Time:

10 working days


Shipment Requirement:

Keep specimen at room temperature. DO NOT ship on ice or dry ice or expose to direct sunlight.

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