ONCODEcipher Colon Essential67 + MSI Panel
FFPE DNA panel targeting 67 relevant genetic mutations in colon cancer AND microsatellite instability (MSI) status to provide treatment options & prognostic indications.
Highlighted Genes -- Mutations of RAS/ RAF/ MEK/ MAP kinase signalling pathway
Next Generation Sequencing (NGS) AND
Polymerase Chain Reaction (PCR) & Fragment Analysis
Essential 67 NGS Panel
DNA extracted from FFPE tissue is subjected to amplification-based library preparation and massively parallel sequencing. Sequencing analysis is performed using commercial clinical bioinformatics pipelines and annotation is done using proprietary and public databases.
MSI PCR & Fragment Analysis Panel
DNA extracted from FFPE tissue (tumour) & blood (non-tumour) is subjected to PCR & amplicons are separated and identified by fragment analysis. The pattern of amplicons for the 5 mononucleotide markers is compared between tumour and non-tumour.
FFPE tissue (FFPE block or 10-15 unstained slides [10µm thickness] with minimal surface area of 5mm2 and at least 30% tumour cellularity) & 1 H&E stained slide
AND 3ml Blood in EDTA tube(s)
Turn Around Time:
10 working days
Keep specimen at room temperature. DO NOT ship on ice or dry ice or expose to direct sunlight.