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ONCODEcipher Microsatellite Instability (MSI) PCR Panel

Product Description:

PCR and fragment analysis-based assay targeting MSI to detect the increase in length of the microsatellite segment by comparing microsatellite allele size variations between normal and tumour samples in five mononucleotide repeat markers for the discovery of treatment options such as immunotherapy.

Target Markers -- NR-21, NR-24, BAT-26, BAT-25, & MONO-27


Polymerase Chain Reaction (PCR) & Fragment Analysis

Test Methodology:

DNA extracted from FFPE tissue (tumour) & blood (non-tumour) is subjected to PCR & amplicons are separated and identified by fragment analysis. The pattern of amplicons for the 5 mononucleotide markers is compared between tumour and non-tumour.

Specimen Requirements:

FFPE tissue (FFPE block or 5 unstained slides [10µm thickness] with minimal surface area of 5mm2 and at least 30% tumour cellularity) & 1 H&E stained slide
AND 3ml Blood in EDTA tube(s)


Turn Around Time:

10 working days


Shipment Requirement:

Keep specimen at room temperature. DO NOT ship on ice or dry ice or expose to direct sunlight.

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