ONCODEduce MPN PCR Panel
Qualitative PCR based panel to detect the presence of JAK2 V617F, JAK2 exon 12, Calreticulin and MPL W515K/L/A & S505N mutations which is useful in the assessment of Myeloproliferative conditions.
Genes Covered-- JAK2 V617F & exon 12, CALR, MPL W515K/L/A & S505N mutations
Polymerase Chain Reaction (PCR)
DNA extracted from blood/ marrow is subjected to PCR with primers targeting these hotspot regions and/or variants. JAK2 exon 12 & CALR amplicons are separated by capillary electrophoresis and identified by label and size, while JAK2 V617F & MPL amplicons are separated by agarose gel electrophoresis and identified by size.
3ml of Blood or Marrow Aspirate in EDTA tube
Turn Around Time:
15 working days
Keep specimen at room temperature. DO NOT ship on ice or dry ice or expose to direct sunlight.