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OUR TECHNOLOGY

PCR and Fragment Analysis

A technique used to amplify a specific piece of DNA or RNA from a sample.

Polymerase Chain Reaction (PCR) is a technique used to amplify a specific piece of DNA or RNA from a sample. PCR can be used to detect certain chances in gene or chromosome. Fragment analysis is a genetic marker analysis which rely on the detection of the changes in the length of a specific DNA sequence to indicate the presence of absence of a genetic marker.

How does Fragment Analysis Works? [4]

NGS is able to detect ALL known variants and any actionable gene mutations (with established targeted therapy drug available). It helps to:


• Avoid unnecessary ‘trial and error’ of medication, unnecessary cost and waste of time trying to find the drugs that work
• Identify ‘drugg-able’ mutations and treat with the right drug
• Detect genetic changes with high sensitivity (>99% on targets) and accuracy (100% coverage)
• Monitor disease progression through retrievable personal database in Oncode
• Identify family risk for cancers if indicated

Step 1

Labeling fragments with fluorescent dyes

Step 2

Amplification of labeled fragments using PCR

Step 3

Separating the fragments by size using Capilary Electrophoresis (CE)

Step 4

Data analysis using software

View all cited references

OUR TECHNOLOGY

PCR and Fragment Analysis

A technique used to amplify a specific piece of DNA or RNA from a sample.

Polymerase Chain Reaction (PCR) is a technique used to amplify a specific piece of DNA or RNA from a sample. PCR can be used to detect certain chances in gene or chromosome. Fragment analysis is a genetic marker analysis which rely on the detection of the changes in the length of a specific DNA sequence to indicate the presence of absence of a genetic marker.

How does Fragment Analysis Works? [4]

NGS is able to detect ALL known variants and any actionable gene mutations (with established targeted therapy drug available). It helps to:


• Avoid unnecessary ‘trial and error’ of medication, unnecessary cost and waste of time trying to find the drugs that work
• Identify ‘drugg-able’ mutations and treat with the right drug
• Detect genetic changes with high sensitivity (>99% on targets) and accuracy (100% coverage)
• Monitor disease progression through retrievable personal database in Oncode
• Identify family risk for cancers if indicated

Step 1

Labeling fragments with fluorescent dyes

Step 2

Amplification of labeled fragments using PCR

Step 3

Separating the fragments by size using Capilary Electrophoresis (CE)

Step 4

Data analysis using software

View all cited references
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